cd133 expression (Miltenyi Biotec)
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cd133 expression
Cd133 Expression, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd133+expression/Prominin-1+Antibody%2C+anti-mouse/pm40543657-214-5-28
Average 96 stars, based on 23 article reviews
Cd133 Expression, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd133+expression/Prominin-1+Antibody%2C+anti-mouse/pm40543657-214-5-28
Average 96 stars, based on 23 article reviews
cd133 expression - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: Alteration in stemness causes exclusivity between Epstein-Barr virus-positivity and microsatellite instability status in gastric cancer. Article Snippet: Each GC cell was incubated with TrypLE Express (Gibco, Carlsbad, CA, USA) for 10 min for detachment, with minimal loss of surface protein expression [16] . .. After washing with cold incubation buffer (comprised of PBS, 0.5% bovine serum albumin, and 0.05% sodium azide), cells were incubated on ice with antibodies anti-CD44 (1:50, ab81424, Abcam) or anti-CD133 (1:10, 130-098-826, Miltenyi Biotec, Germany) for 30 min. FACSCanto II System (BD Biosciences, San José, CA, USA) was used to detect CD44 and Expressing:Article Title: Alteration in stemness causes exclusivity between Epstein-Barr virus-positivity and microsatellite instability status in gastric cancer. Article Snippet: Each GC cell was incubated with TrypLE Express (Gibco, Carlsbad, CA, USA) for 10 min for detachment, with minimal loss of surface protein expression [16] . .. After washing with cold incubation buffer (comprised of PBS, 0.5% bovine serum albumin, and 0.05% sodium azide), cells were incubated on ice with antibodies anti-CD44 (1:50, ab81424, Abcam) or anti-CD133 (1:10, 130-098-826, Miltenyi Biotec, Germany) for 30 min. FACSCanto II System (BD Biosciences, San José, CA, USA) was used to detect CD44 and Article Title: Glutamine deprivation in glioblastoma stem cells triggers autophagic SIRT3 degradation to epigenetically restrict CD133 expression and stemness. Article Snippet: 1 Department of Biochemistry & Molecular Cell Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai, China 2 College of Life Sciences, Shaanxi Normal University, Xi’an, Shaanxi, China 3 School of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China 4 Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China 5 Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Department of Biochemistry & Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China Abstract Glioblastoma multiforme (GBM) is a highly malignant brain tumor, and glioblastoma stem cells (GSCs) are the primary cause of GBM heterogeneity, invasiveness, and resistance to therapy.. Sirtuin 3 (SIRT3) is mainly localized in the mitochondrial matrix and plays an important role in maintaining GSC stemness through cooperative interaction with the chaperone protein tumor necrosis factor receptor-associated protein 1 (TRAP1) to modulate mitochondrial respiration and oxidative stress.. The present study aimed to further elucidate the specific mechanisms by which SIRT3 influences GSC stemness, including whether SIRT3 serves as an autophagy substrate and the mechanism of SIRT3 degradation. Article Title: Cilium induction triggers differentiation of glioma stem cells. Article Snippet: Typically, GSCs are characterized by forming spheres expressing stem-cell markers, such as CD133, sex-determining region Y-box 2 (Sox2), Musashi, and Nestin. .. Article Title: The Long Non-coding RNA HOTAIR Controls the Self-renewal, Cell Senescence, and Secretion of Anti-aging Protein α-Klotho in Human Adult Renal Progenitor Cells. Article Snippet: The long non-coding RNAs (lncRNA) play an important role in several biological processes, including some renal diseases.. Nevertheless, little is known about lncRNA that are expressed in the healthy kidneys and involved in renal cell homeostasis and development, and even less is known about lncRNA involved in the maintenance of human adult renal stem/progenitor cells (ARPCs) that have been shown to be very important for renal homeostasis and repair processes.. Through a whole-genome transcriptome screening, we found that the HOTAIR lncRNA is highly expressed in renal progenitors and potentially involved in cell cycle and senescence biological processes. Article Title: Expansion of circulating stem-like CD8 + T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in mice Article Snippet: The B16F10 cell line was provided by Prof. Hanspeter Pircher, (Medical Center – University of Freiburg, Germany) and transduced with lentiviral particles encoding the human stem cell marker CD133, and sorted for CD133 expression using the CD133 MicroBead Kit (Miltenyi Biotec). .. The B16F10 cell line was provided by Prof. Hanspeter Pircher, (Medical Center – University of Freiburg, Germany) and transduced with lentiviral particles encoding the human stem cell marker CD133, and sorted for Article Title: Glutamine deprivation in glioblastoma stem cells triggers autophagic SIRT3 degradation to epigenetically restrict CD133 expression and stemness Article Snippet: .. CD133+ GSCs were sorted by magnetic bead separation based on Article Title: PDPN marks a subset of aggressive and radiation-resistant glioblastoma cells Article Snippet: .. FACSAria (BD Biosciences) was used to analyze and sort GSCs based on PDPN and Article Title: An old spice with new tricks: Curcumin targets adenoma and colorectal cancer stem-like cells associated with poor survival outcomes. Article Snippet: Cell culture, in vitro methods and biophysical assays Full details on ex vivo spheroid and 2D-cell culture and incubation with curcumin are provided in Supplementary materials, along with descriptions of the protein pull-down methodology and biophysical assays. .. To assess ALDH activity and Control:Article Title: Cilium induction triggers differentiation of glioma stem cells. Article Snippet: Typically, GSCs are characterized by forming spheres expressing stem-cell markers, such as CD133, sex-determining region Y-box 2 (Sox2), Musashi, and Nestin. .. FACS:Article Title: The Long Non-coding RNA HOTAIR Controls the Self-renewal, Cell Senescence, and Secretion of Anti-aging Protein α-Klotho in Human Adult Renal Progenitor Cells. Article Snippet: The long non-coding RNAs (lncRNA) play an important role in several biological processes, including some renal diseases.. Nevertheless, little is known about lncRNA that are expressed in the healthy kidneys and involved in renal cell homeostasis and development, and even less is known about lncRNA involved in the maintenance of human adult renal stem/progenitor cells (ARPCs) that have been shown to be very important for renal homeostasis and repair processes.. Through a whole-genome transcriptome screening, we found that the HOTAIR lncRNA is highly expressed in renal progenitors and potentially involved in cell cycle and senescence biological processes. Transduction:Article Title: Expansion of circulating stem-like CD8 + T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in mice Article Snippet: The B16F10 cell line was provided by Prof. Hanspeter Pircher, (Medical Center – University of Freiburg, Germany) and transduced with lentiviral particles encoding the human stem cell marker CD133, and sorted for CD133 expression using the CD133 MicroBead Kit (Miltenyi Biotec). .. The B16F10 cell line was provided by Prof. Hanspeter Pircher, (Medical Center – University of Freiburg, Germany) and transduced with lentiviral particles encoding the human stem cell marker CD133, and sorted for Marker:Article Title: Expansion of circulating stem-like CD8 + T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in mice Article Snippet: The B16F10 cell line was provided by Prof. Hanspeter Pircher, (Medical Center – University of Freiburg, Germany) and transduced with lentiviral particles encoding the human stem cell marker CD133, and sorted for CD133 expression using the CD133 MicroBead Kit (Miltenyi Biotec). .. The B16F10 cell line was provided by Prof. Hanspeter Pircher, (Medical Center – University of Freiburg, Germany) and transduced with lentiviral particles encoding the human stem cell marker CD133, and sorted for Activity Assay:Article Title: An old spice with new tricks: Curcumin targets adenoma and colorectal cancer stem-like cells associated with poor survival outcomes. Article Snippet: Cell culture, in vitro methods and biophysical assays Full details on ex vivo spheroid and 2D-cell culture and incubation with curcumin are provided in Supplementary materials, along with descriptions of the protein pull-down methodology and biophysical assays. .. To assess ALDH activity and |
